cdk6 cell signaling Search Results


mouse monoclonal antibodies against cdk6  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc mouse monoclonal antibodies against cdk6
FIGURE 4 | Hypoxia and co-culture with bone marrow mesenchymal stromal cells (BMSC) + TGFβ-1 increases the expression of markers of leukemia cell quiescence. KG1a cells were cultured alone or with alginate-encapsulated BMSC and TGFβ-1 (10 ng/ml) over a 12-day period in normoxia or hypoxia (1% pO2). (A) Expression of CD150 determined in the live fraction by flow cytometry. MFI, mean fluorescent intensity. *p < 0.05 (Student’s t-test). (B–E) Results of quantitative RT-PCRs on RNA isolated on days 8 and 12. *p < 0.05 (one-way ANOVA). (F) Gene expression analysis of p21 expression conducted on the GSE17054 transcriptomic dataset. The graph represents the changes in gene expression between hematopoietic stem cells and leukemic stem cells. *p < 0.05 (Welch’s t-test). (G) Western blot images for the expression of p21, CDK4, and <t>CDK6</t> conducted on samples isolated at day 12 of culture. SN, single culture at normoxia; CH, co-culture under hypoxia.
Mouse Monoclonal Antibodies Against Cdk6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal antibodies against cdk6/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
mouse monoclonal antibodies against cdk6 - by Bioz Stars, 2026-03
96/100 stars
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cdk6  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc cdk6
FIGURE 4 | Hypoxia and co-culture with bone marrow mesenchymal stromal cells (BMSC) + TGFβ-1 increases the expression of markers of leukemia cell quiescence. KG1a cells were cultured alone or with alginate-encapsulated BMSC and TGFβ-1 (10 ng/ml) over a 12-day period in normoxia or hypoxia (1% pO2). (A) Expression of CD150 determined in the live fraction by flow cytometry. MFI, mean fluorescent intensity. *p < 0.05 (Student’s t-test). (B–E) Results of quantitative RT-PCRs on RNA isolated on days 8 and 12. *p < 0.05 (one-way ANOVA). (F) Gene expression analysis of p21 expression conducted on the GSE17054 transcriptomic dataset. The graph represents the changes in gene expression between hematopoietic stem cells and leukemic stem cells. *p < 0.05 (Welch’s t-test). (G) Western blot images for the expression of p21, CDK4, and <t>CDK6</t> conducted on samples isolated at day 12 of culture. SN, single culture at normoxia; CH, co-culture under hypoxia.
Cdk6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdk6/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
cdk6 - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier

Image Search Results


FIGURE 4 | Hypoxia and co-culture with bone marrow mesenchymal stromal cells (BMSC) + TGFβ-1 increases the expression of markers of leukemia cell quiescence. KG1a cells were cultured alone or with alginate-encapsulated BMSC and TGFβ-1 (10 ng/ml) over a 12-day period in normoxia or hypoxia (1% pO2). (A) Expression of CD150 determined in the live fraction by flow cytometry. MFI, mean fluorescent intensity. *p < 0.05 (Student’s t-test). (B–E) Results of quantitative RT-PCRs on RNA isolated on days 8 and 12. *p < 0.05 (one-way ANOVA). (F) Gene expression analysis of p21 expression conducted on the GSE17054 transcriptomic dataset. The graph represents the changes in gene expression between hematopoietic stem cells and leukemic stem cells. *p < 0.05 (Welch’s t-test). (G) Western blot images for the expression of p21, CDK4, and CDK6 conducted on samples isolated at day 12 of culture. SN, single culture at normoxia; CH, co-culture under hypoxia.

Journal: Frontiers in cell and developmental biology

Article Title: Recreating the Bone Marrow Microenvironment to Model Leukemic Stem Cell Quiescence.

doi: 10.3389/fcell.2021.662868

Figure Lengend Snippet: FIGURE 4 | Hypoxia and co-culture with bone marrow mesenchymal stromal cells (BMSC) + TGFβ-1 increases the expression of markers of leukemia cell quiescence. KG1a cells were cultured alone or with alginate-encapsulated BMSC and TGFβ-1 (10 ng/ml) over a 12-day period in normoxia or hypoxia (1% pO2). (A) Expression of CD150 determined in the live fraction by flow cytometry. MFI, mean fluorescent intensity. *p < 0.05 (Student’s t-test). (B–E) Results of quantitative RT-PCRs on RNA isolated on days 8 and 12. *p < 0.05 (one-way ANOVA). (F) Gene expression analysis of p21 expression conducted on the GSE17054 transcriptomic dataset. The graph represents the changes in gene expression between hematopoietic stem cells and leukemic stem cells. *p < 0.05 (Welch’s t-test). (G) Western blot images for the expression of p21, CDK4, and CDK6 conducted on samples isolated at day 12 of culture. SN, single culture at normoxia; CH, co-culture under hypoxia.

Article Snippet: After blocking, the blots were incubated with rabbit monoclonal antibodies against CDK4 [Cell Signaling Technology (CST)] and p21 (Santa Cruz) and with mouse monoclonal antibodies against CDK6 (CST) and actin (Sigma-Aldrich).

Techniques: Co-Culture Assay, Expressing, Cell Culture, Cytometry, Isolation, Gene Expression, Western Blot